庄小威，1972年生人。1987年，15岁时从苏州中学科大少年班预备班考入中国科技大学少年班。
Nanoscopic Optical Imaging
Optical microscopy is one of the most widely used imaging methods in biomedical research. Several advantages make light microscopy a particularly powerful tool for cell, tissue and animal imaging. These include the exquisite molecular specificity, the relatively fast time resolution and the non-invasive imaging nature. However, the spatial resolution of far-field optical microscopy, classically limited by the diffraction of light to a few hundred nanometers, is substantially larger than typical molecular length scales in cells. This limit leaves many biological problems beyond the reach of light microscopy. To overcome this limit, we have developed a new form of super- resolution light microscopy, stochastic optical reconstruction microscopy (STORM). STORM uses photo-switchable fluorescent probes to temporally separate the otherwise spatially overlapping images of individual molecules, allowing the construction of high-resolution images. Using this concept, we have achieved three-dimensional, multicolor fluorescence imaging of molecular complexes, cells, and tissues with ~20 nm lateral and ~50 nm axial resolutions. We hope to advance STORM capabilities to ultimately enable real-time imaging of cells and tissues with resolution at the true molecular length scale. This new form of fluorescence microscopy allows molecular interactions in cells and cell-cell interactions in tissues to be imaged at the nanometer scale. We are applying this new technology to cell biology and neurobiology.
